Methods and Instruments
I. LC-ESI-MS-Kopplung zur Detektion, Strukturaufklärung und Quantifizierung von Metaboliten
1. LC-ESI-QqQ-Massenspektrometrie (Agilent 6460) | |
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2. LC-QqTOF-Massenspektrometrie (Agilent 96520A) | |
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II. GC-EI-MS Kopplung (Agilent 7890A/5975C) zum "Metabolic Profiling" in Pflanzen, Tieren und Mikroorganismen
As a routine method we are profiling relative concentration changes in primary metabolites (sugars, sugar-alcohols, amino acids, organic acids and phosphorylated compounds). The mainly polar metabolites are extracted from the tissue, derivatised and the derivates are quantified and identified with an Agilent GC-Quadrupole-MS System, which can operate in EI and CI mode. Several more targeted methods for analysing particular (also non-polar) compounds are developed in cooperation with the various users. |
Fig.1: Example Chromatogram for a GC-MS primary metabolite profiling in Physcomitrella patens
Fig.2: Example for a cluster analysis for the relative changes of metabolite concentrations in Ricinus communis under anoxia.