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Projects

Projects

Running projects

IGEM Freiburg 2015 – DIA-Chip

The Freiburg IGEM student team 2015 decided to use the microarray copying approach of our group to copy disease markers from a DNA microarray to a protein microarray. Based on our label-free detection device they like to analyse blood samples for marker-binding antibodies to conclude if the blood sample was exposed and/or infected with the according disease. The AG Roth is happy to support the IGEM team and crosses fingers for the Boston final in September.

Partners:

ReelinSys - Systems biology of Reelin-associated neuropsychiatric disorders
(FKZ 0316174C, BMBF e:bio)

Reelin is a central molecule in neuronal signalling and development. Reelin changes in concentration or mutations may lead to severe neuropsychiatric disorders. ReelinSys therefore aims

  • to understand the Reelin signalling network and its behaviour
  • to understand Reelin-associated neuropsychiatric disorders

The integrative dynamic mechanistic Reelin network will enable

  • the identification of novel neuropsychiatric drug targets
  • the definition of desired biological effects or toxicity pathways of currently used and potential novel neuropsychiatric drugs

AG Roth aims to copy several major biomolecular players from the Reelin pathway onto and into a microarray and to analyse the dynamics and the interaction kinetics of these pathway molecules with each other. Resulting Kd-values, on- and off-rates will generate an additional level of information enabling better simulations for Reelin pathway behaviour and dynamics.

Partners:

Protein Kopierer – Aufbau eines Gerätes zur Erzeugung von Protein-Mikroarrays als Kopien eines DNA-Mikroarrays
(FKZ 7533-7-11.6.3, MWK Ideenwettbewerb BaWü 2010)

Ideenwettbewerb BaWü 2010 aimed for novel technologies in the area of medicine, health and materials. Jürgen Burgers PhD topic to generate protein microarrays as copies of DNA microarrays was awarded first with an “evaluation price” among 30 others and in a second round was awarded with a “development price” to realize a protein microarray copier.

Partners:

CE microarray – Realization of a novel micro plate reader device to detect earlier and better sepsis
(funding number 606618, EU FP7 - Innovation)

Sepsis is a ife-threatening illness caused by the body’s overreaction to an infection and can be triggered either directly by infection or may occur after medical treatment or surgery. The mortality rate in patients admitted to hospital with severe sepsis is 28-50% and it remains the most prevalent cause of death in non-coronary Intensive Care Units (ICUs ). Therefore it is essential to go novel ways for sepsis detection to be faster and more sensitive in detection. Thus a novel microplate reader with 3 to 10-fold sensitivity is evaluated together with a sepsis marker panel.

AG Roth aims to validate these markers and the according antibodies for specificity, sensitivity and cross-reactivity in terms of kinetics like on- and off-rates, cause a fully automated sepsis marker panel should be processed for all antibodies in parallel in an identical way, meaning that all antibodies applied should have roughly the same on- and off-rates to have in all cases a minimum of background and a maximum of signal. We will provide calibrator solutions and antibody kinetics.

Partners:

Former projects

SystemCerv – Systems biology approaches to cervical pre-cancer and cancer
(funding number 306037, EU FP7 - health)

Currently cervical histology is the gold standard procedure in the field. However, there is a need for alternative approaches and specific biomarkers to aid objective CIN lesion grading and to identify true high grade cervical disease, especially in the advent of primary HPV screening and widespread HPV vaccination. Therefore a novel marker panel shall be derived by systems biology and tested by phage display derived antibodies.

AG Roth realized in this project the measurement and analysis of antibody kinetics as well as specificity and sensitivity. We build up a device capable for label-free analysis of binding and interaction kinetics.

Partners:

Contact

Center for Biological Systems Analysis

University of Freiburg

 

  • Address:

    Habsburgerstr. 49
    79104 Freiburg
    Germany
 
  • Delivery entrance:

    Hauptstr. 1
    79104 Freiburg
 

 

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