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Methods and Instruments

Instrumente, Methoden und Anwendungen

 

 

 

Instruments

 

 

 

MS

 

  • Agilent 6340 ETD Ion Trap
  • Agilent 6460 Triple Quadrupole
  • Agilent 6520 Q-TOF

all Agilent MS are online coupled to a nanoChip-HPLC System

MS

 

  • Thermo LTQ-Orbitrap XL

 

online coupled to nano-HPLC (Agilent 1200 Series)

 

 

 

HPLC

 

  • Agilent 1200 Series with fraction collector
  • Dionex Ultimate 3000 (Titanium) with Probot fraction collector

 

 

 

Elektrophoresis

 

  • Elektrophoresis
  • Agilent OFFGEL-Elektrophoresis
  • ETC horizontal gel-elektrophoresis (Flat Top)
  • BioRad 1D-SDS-Page
  • Invitrogen 1D-SDS-Page
Elektrophorese

 

 

 

Laser-Scanner

 

  • GE Typhoon 9400

 

 

 

Software

 

  • Mascot Server
  • Mascot Distiller (Quantitation Toolbox)
  • Mascot Integra
  • Scaffold 4.0
  • PEAKS
  • MaxQuant
Software

 

 

 

Methods

 

  • nanoLC-MS/MS
  • Phosphopeptide enrichment using TiO2-affinty column (Ultimate 3000)
  • Electron Transfer Dissociation (ETD)
  • Multiple Reaction Monitoring (MRM)
  • Metabolic Labeling (15N und SILAC)

 

 

 

Tasks

 

  • Protein ID
  • De novo Sequencing
  • post translational modification (PTM) analysis
  • Determination of intact protein masses
  • Identification of protein interactions
  • Quantitativ Analysis
software2

 

 

 

 

 


 
Contact

Center for Biological Systems Analysis

University of Freiburg

 

  • Address:

    Habsburgerstr. 49
    79104 Freiburg
    Germany
 
  • Delivery entrance:

    Hauptstr. 1
    79104 Freiburg
 

 

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